Studg on radioimmunoassay of FDP

DOI
  • OUMI Naohito
    Department of Medicine, Cancer Reserch Institute, Sapporo Medical Callege
  • KOGO Yutaka
    Department of Medicine, Cancer Reserch Institute, Sapporo Medical Callege
  • NIITSU Yoshihiro
    Department of Medicine, Cancer Reserch Institute, Sapporo Medical Callege
  • URUSHIZAKI Ichiro
    Department of Medicine, Cancer Reserch Institute, Sapporo Medical Callege

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Other Title
  • FDPの radioimmunoassay 法に関する研究

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Description

Fibrinogen degradation products, fragment D (FgD) and fragment E (FgE) were measured in human serum by an radioimmunoassay (RIA) technique.<br>Fibrinogen was digested for five hours by plasmin to obtain FgD and FgE as the major products.<br>Techniques for separating these fragments from each other and from larger fragments (FgX, FgY) present in the crude digest, comprise gelfiltration with Sephadex G-200 and isoelectric fractionation which gave satisfactory purity and efficiency for preparative purpose.<br>Fragments were iodinated by chloramin T method, and separation of antibody bound and free antigen was achieved using second antibody.<br>In eighteen healthy subjects, the serum level of FgE ranged from 104 to 206ng/ml and raised levels were found in the patients with disseminated intravascular coagulation in the course of acute promyelocytic leukemias and gastric cancers.<br>Advantages of RIA system over the tanned red cell haemagglutination inhibition immunoassay was its higher sensitivity (20ng/ml)<br>There is, however, a possibility that RIA of of FgDP is detecting residual fibrinogen in the serum, since antiFgDP antiserum showed weak cross-reaction to fibrinogen by double immunodiffusion test.<br>In order to eliminate interference by residual fibrinogen in the serum, antiserum against FgDP was further purified by affinity chromatography to remove antifibrinogen activity.<br>A radioimmunoassay, using this FgDP specific antiserum is now under investigation.

Journal

  • Blood & Vessel

    Blood & Vessel 9 (1), 65-71, 1978

    The Japanese Society on Thrombosis and Hemostasis

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