か粒球由来の抗凝固活性をもつ塩基性蛋白　　内毒素との結合性およびその機作について

DOI

書誌事項

タイトル別名
  • Anticoagulant, cationic protein (CAP) isolated from granulocytes: Endotoxin-binding property of CAP.
  • Endotoxin-binding property of CAP
  • 内毒素との結合性およびその機作について

抄録

Prior incubation of endotoxin with cationic protein (CAP) from mouse bone marrow or spleen cells has been shown to inhibit some endotoxin activities such as tissue factor generation in spleen cells and lethality in mice.<br>In this study, the binding mechanism of CAP isolated from rabbit granulocytes to endotoxin was investigated.<br>1) CAP significantly prolonged the clotting time (prothrombin time) of human plasma in proportion to the amount of CAP added. 2) Binding of CAP to Reendotoxin was shown to form an insoluble complex since addition of CAP solution to Reendotoxin increased turbidity. 3) The binding of CAP to Re-endotoxin was optimal under the condition of low ionic strength. About 50% of the binding was inhibited by the addition of 200mM-NaCl. 4) The binding of CAP to Re-endotoxin or to lipid A was best expressed at pH 6.0 to 7.0. 5) Rapid increase of turbidity was observed in the mixture of CAP and Re-endotoxin or lipid A. 6) Binding of CAP to Re-endotoxin was reversible.<br>These findings suggest that CAP binds to endotoxin through the ionic and hydrophobic bonds, and results in the inactivation of endotoxin. DIC manifestation in endotoxemia may be regulated by CAP.

収録刊行物

  • 血液と脈管

    血液と脈管 18 (6), 592-594, 1987

    一般社団法人 日本血栓止血学会

キーワード

詳細情報 詳細情報について

  • CRID
    1390282679836640512
  • NII論文ID
    130004261276
  • DOI
    10.2491/jjsth1970.18.592
  • ISSN
    18842372
    03869717
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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