血管内凝固とプレカリクレイン

DOI オープンアクセス

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タイトル別名
  • Changes of prekallikrein in the cases with DIC syndrome

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説明

The characteristics of the trigger substances of disseminated intravascular coagulation syndrome in the cases with gastric cancer and acute promyelocytic leukemia, and those of the serine proteases (Factor Xa, thrombin, plasmin and trypsin) were studies from the viewpoints of the prekallikrein activation.<br>The serine proteases and antithrombin III were purified by our methods, and prekallikrein was assayed by our method.<br>Results:<br>(1). Endotoxin (E. Coli, Difco Co., 0.2mg/ml in the final concentration) activated factor XII in the citrated plasma to show the shortened PTT, and the factor XIIa activated prekallikrein in the plasma. Kallikrein activated the purified factor VII from 25% to 47% compared with the normal plasma. Ten mg/ml of endotoxin in the plasma activated coagulation system to show 0.04units/ml of factor Xa and 0.025units/ml of thrombin assayed by the methods using synthetic chromogenic substrates.<br>(2). 6×104/cmm of the cultured well differentiated adenocarcinoma cells and the pathologic cells in the case with acute promyelocytic leukemia activated 1μg of the purified bovine prekallikrein.<br>(3). Two units/ml of factor Xa, 20units/ml of plasmin and 0.03mg/ml of trypsin activated the prekallikreinat 10min activation time; Factor Xa: 25%, plasmin: 15%, and trypsin: 41%.<br>(4). The purified antithrombin III inhibited kallikrein, and heparin accelerated this inhibitory effect. Aprotinin (500units/ml in the final concentration) inhibited 85% prekallikrein activation by addition of kaolin. Dextran sulfate (0.3 and 3mg/ml in the final cocentration) showed the antithrombin activity, and inhibited prekallikrein activation by addition of kaolin; 0.3mg/ml: 78% and 3mg/ml: 64%.

収録刊行物

  • 血液と脈管

    血液と脈管 10 (4), 639-642, 1979

    一般社団法人 日本血栓止血学会

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