Glycoconjugate histochemistry and ultrastructural study of membranous lipodystrophy. A case report.

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A biopsy case of a 40-year old housewife, who was admitted to the hospital for bilateral elbow joint pain is examined. Light and electron microscopic examination of the biopsied material from the cystic lesion of her right humerus revealed a membranocystic lesion which was characteristic of membranous lipodystrophy (21).<br>Histochemical studies using lectin-HRP conjugates provided precise information on the carbohydrate components of the membranocystic lesion. MPA, specific for α-D-galactose residues, strongly stained the membrane of the membranocystic lesion, whereas, RCA-I and SBA, specific for β-D-galactose residues, did not bind the membrane. WGA and HPA failed to stain the membrane of the typical membranocystic lesion, but bound the degenerated adipose cells of the bone marrow.<br>The morphogenesis and the glycoconjugates histochemistry of the membranocystic lesion are discussed in the present paper.

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