MICROANALYSIS OF MITOCHONDRIAL GRANULES. MICROANALYSIS OF MITOCHONDRIAL GRANULES IN BONE CELLS INCIDENT TO EXPERIMENTAL TOOTH MOVEMENT

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  • HIRASHITA AYAO
    Department of Orthodontics, School of Dental Medicine, Tsurumi University
  • NAKAMURA YOSHIKI
    Department of Orthodontics, School of Dental Medicine, Tsurumi University
  • OKUMURA EIJI
    Department of Orthodontics, School of Dental Medicine, Tsurumi University
  • KUWABARA YOSUKE
    Department of Orthodontics, School of Dental Medicine, Tsurumi University

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Description

Molars of adult Wistar rats were moved experimentally by orthodontic elastic for four days. Then, energy dispersive X-ray microanalysis of mitochondrial granules in bone cells incidental to experimental tooth movement was performed. The following results were obtained. 1) In the unstained specimens treated with OsO4-glutaraldehyde, microanalysis seems to be possible for a portion of the elements. 2) In particular, a condition in which Ca may bind strongly with protein in mitochondria is shown. 3) At mineralizing sites in the experimental groups, Ca-K, P-K, and Os-M X-ray lines were detected in mitochondrial granules in morphologically active osteoblasts and young osteocytes, but we could not separate the P-K and Os-M peaks from their combined peak. At absorbing mineral sites, Ca-K was found markedly in the ruffled borders and vacuoles of osteoclasts. Ca-K X-ray line was detected also in mitochondrial granules of osteoclasts. 4) In the control groups, mitochondria of resting osteoblasts and osteocytes had few granules and the detection of Ca-K X-ray line was very hard from these mitochondria. Ca-K X-ray line was detected in the mitochondria close to the ruffled border of the osteoclasts, but it was not detected in the mitochondria located on the vascular side of the cells.

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