A STUDY OF POTASSIUM-OSMIUM-CYANIDE COMPLEX

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  • HOSHINO YOSHINOBU
    The Department of Research Oncology and Cell Biology, Fuchu Metropolitan Hospital The Department of Surgery, Sinai Hospital of Baltimore, The Johns Hoplins University School of Medicine
  • SHANNON, Jr. W. ALLEN
    The Department of Research Oncology and Cell Biology, Fuchu Metropolitan Hospital The Department of Surgery, Sinai Hospital of Baltimore, The Johns Hoplins University School of Medicine Present Address: Research Morphology and Cytochemistry Unit, General Medical Research, V. A. Hospital
  • SELIGMAN ARNOLD M.
    The Department of Research Oncology and Cell Biology, Fuchu Metropolitan Hospital The Department of Surgery, Sinai Hospital of Baltimore, The Johns Hoplins University School of Medicine Research Oncology and Cell Biology

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  • APPLICATION TO ULTRASTRUCTURAL CYTOCHEMISTRY WITH DIAMINOBENZIDINE

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Potassium-osmium-cyanide complex (POCC) was substituted for osmium tetroxide procedures for the ultrastructural demonstration of cytochrome oxidase and peroxidase with diaminobenzidine (DAB).<br>POCC reacted with DAB polymer resulting from the oxidation reaction producing electron opaque deposits. POCC produced fine homogeneous staining and more contrast than did the usual osmication method. This suggests a means of making the methods more sensitive. Careful controls are necessary since POCC alone stains smooth membrane elements, thereby possibly obscuring true reaction products in some cases.

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