Characterization of a Novel Monoclonal Antibody against Human Mitochondrial Ferritin and Its Immunohistochemical Application in Human and Monkey Substantia Nigra
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- Yang Mingchun
- Molecular Neuroscience Research Center, Shiga University of Medical Science Department of Neurosurgery, 1st Affiliated Hospital, Harbin Medical University
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- Yang Hongkuan
- Molecular Neuroscience Research Center, Shiga University of Medical Science Department of Neurosurgery, 1st Affiliated Hospital, Harbin Medical University
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- Guan Hongpeng
- Molecular Neuroscience Research Center, Shiga University of Medical Science Department of Neurosurgery, 1st Affiliated Hospital, Harbin Medical University
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- Sugihara Hiroyuki
- Department of Pathology, Shiga University of Medical Science
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- Terada Tomohiro
- Department of Pharmacy, Shiga University of Medical Science
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- Tooyama Ikuo
- Molecular Neuroscience Research Center, Shiga University of Medical Science
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- Kato Tomoko
- Molecular Neuroscience Research Center, Shiga University of Medical Science
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- Mukaisho Kenichi
- Department of Pathology, Shiga University of Medical Science
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- Ogasawara Kazumasa
- Department of Pathology, Shiga University of Medical Science
書誌事項
- 公開日
- 2017
- DOI
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- 10.1267/ahc.16034
- 公開者
- 日本組織細胞化学会
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説明
<p>Mitochondrial ferritin (FtMt) is a novel iron storage protein with high homology to H-ferritin. Unlike the ubiquitously expressed H- and L-ferritin, FtMt is expressed in specific tissues such as the testis, heart, and brain. The function of FtMt is not fully understood; however, evidence suggests that it has a neuroprotective role in neurodegenerative diseases. We have previously reported that FtMt is expressed in catecholaminergic neurons of the monkey brainstem. To explore FtMt expression in human dopaminergic neurons, we designed a novel monoclonal antibody, C65-2, directed against human FtMt. Here, we report the properties of our C65-2 antibody. Western blots analysis and immunoabsorption tests demonstrated that the C65-2 antibody specifically recognized FtMt with no cross-reactivity to H-ferritin. Immunohistochemistry showed that the C65-2 antibody detected FtMt in neurons of the substantia nigra pars compacta (SNc) in humans and monkeys. We confirmed that FtMt is expressed in dopaminergic neurons of the human SNc. Our results suggest that FtMt is involved in various physiological and pathological mechanisms in human dopaminergic neurons, and the C65-2 monoclonal antibody promises to be a useful tool for determining the localization and biological functions of FtMt in the brain.</p>
収録刊行物
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- Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ
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Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 50 (1), 49-55, 2017
日本組織細胞化学会
