ラット馬杉腎炎発症過程における糸球体内凝固・線溶系に関する研究

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タイトル別名
  • The Role of Fibrinolysis and Coagulation in the Glomerulus in the Course of Rat Masugi Nephritis
  • ラット バスギ ジンエン ハッショウ カテイ ニ オケル シキュウ タイナイ
  • -Studies Employing the Isolated Glomeruli-
  • ―単離糸球体を中心としての検討―

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In oder to estimate the role of intraglomerular blood coagulation and fibrinolytis in the course of rat Masugi nephritis, isolated glomeruli were mainly employed in this study. The fibrinolytic activities were examined by the fibrin agar plate and the auther's original method (S-P disc method). By the latter method fibrinolytic activities were determined by counting the radioacti-vities of the degradated products of I-fibrinogen as substrate after the fractionation with SDS-polyacrylamide gel disc electrophoresis. Moreover histological changes were observed with spe-cial reference to the depositions of fibrin or its derivertives.The results obtained were as follows ; (1) Most of the fibrinolytic factors in the glomerulus of the rat were plasminogen activator. Some part of this activator existed in the glomeruli, while the other was easily released. S-P disc method was more advantageous to estimate their activities than F.A.P, method. (2) The difference of the fibrinolytic activity between isolated glomeruli and urokinase was detected in detail by S-P disc method. (3) Depositions of fibrinogen and/or fibrin were detected immunohistologically at the areas of fibrinoid change which was characteristic in the first phase of the rat Masugi nephritis. And the appearance of this change significantly correlated with that of intraglomerular thrombosis and on the contrary showed inverse correlation with glomerular fibrinolytic activities. Moreover the treatment with warfarin reduced the fibrinoid change significantly. (4) In the course of proliferative rat Masugi nephritis in which proteinuria did not appeared in the heterologous phase due to low titer Nephrotoxin injection, marked intraglomerular fibrin depositions were detected in the autologous phase and prior to the appearance of proliferative glomerular changes. At the same time fibrinolytic activity of the glomeruli significantly decreased not only at the period of fibrin depositions but also before then. And more this state of low fibrinolytic activity was maintained in the period of proliferative changes. These findings strongly suggest that the low fibrinolytic activity of the glomeruli which in-duced the hypercoagulable state in conjunction with accelerated blood coagulation process may be one of the important factors in both establishment and maintenance not only of the proliferative changes but also of the non-proliferative (or degenerative) changes in the course of glomerulone-phritis.

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