鉄ニトリロ三酢酸腹腔内投与により発生したラット腎癌からの培養細胞株の樹立とその性状

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  • Establishment and characterization of cell line from ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma

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The characteristics of ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma by intraperitoneal administration and the effects of hormones on the tumor have been studied in vivo. However, to our knowledge there have been no reports on the characteristics of the tumor cells. In this study, we established a tumor cell line from Fe-NTA induced rat renal tumor and investigated tumor cell characteristics. The tumor cell line RKFe was established from Fe-NTA induced rat renal tumor by collagenase digestion. RKFe showed epithelial morphology in culture, and had microvilli and basement membranes under electron microscopy. RKFe cells acquired tolerance to the cytotoxicity of Fe-NTA. They also grew in soft agar culture and formed tumors by the inoculation of cells into nude mice. Histologically, transplanted tumors showed poorly differentiated adenocarcinoma with several tubular structures. A subclone of RKFe, named RKFe-C6, showed metastasis to lungs. The RKFe cell line may be useful for studying the characteristics of Fe-NTA induced rat tumor cells.

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