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- 田中 輝和
- 岡山大学医学部癌源研究施設生化学部門
書誌事項
- タイトル別名
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- Autoradiographic studies on proviral DNA synthesis in enucleated chick embryo fibroblasts infected with avian sarcoma virus
- オートラジオグラフィー ニヨル トリ ニクシュ ウイルス カンセン ダツ カク
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抄録
After infection of RNA tumor viruses to susceptible cells, viral RNA is reversely transcribed into proviral DNA. In order to disclose the site of proviral DNA synthesis in the cells, chick embryo fibroblasts (CEF) were enucleated by centrifugation in the presence of cytochalasin B, and the enucleated CEF (cytoplasts) were infected with B77 strain of avian sarcoma virus (B77-ASV).<br>Incorporation of 3H-thymidine into DNA in the cytoplasts was investigated by autoradiography. Photopositive grains were observed in cytoplasts infected with B77-ASV, but not in mock-infected cytoplasts.<br>The photopositive grains in the cytoplasts infected with B77-ASV disappeared almost completely by DNase I treatment. N-demethyl rifampicin, which is a specific inhibitor of reverse transcriptase, inhibited the appearance of photopositive grains.<br>The B77-ASV-infected cytoplasts were ultrathinsectioned for electron microscopic autoradiography. The photopositive grains appeared in the cytoplasm without relation to mitochondria.<br>These results indicate that the proviral DNA synthesis is initiated in the cytoplasm of B77-ASV-infected chick embryo fibroblast without the direct participation of nucleus.
収録刊行物
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- 岡山医学会雑誌
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岡山医学会雑誌 89 (9-10), 1177-1186, 1977
岡山医学会
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詳細情報 詳細情報について
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- CRID
- 1390282679854634496
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- NII論文ID
- 130006860297
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- NII書誌ID
- AN00032489
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- ISSN
- 18824528
- 00301558
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- NDL書誌ID
- 1913840
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- IRDB
- NDL
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