胆汁色素に関する研究

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書誌事項

タイトル別名
  • Studies on the bile pigment
  • 胆汁色素に関する研究-2-胆汁中Bilirubin異性体の検討
  • タンジュウ シキソ ニカンスルケンキュウ 2 タンジュウチュウ Bilirub
  • 第二編 胆汁中Bilirubin異性体の検討
  • Part 2. Mass spectroscopic analysis of bilirubin IX dimethylester in various liver diseases

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説明

As almost no study has been made on the mass spectroscopic analysis of bilirubin obtained from the bile collected from the patients with liver diseases and Gunn rat, it was attempted to determine the isomeric composition of bilirubins by the use of mass spectrometry. Bilirubin was crystallized from the bile by Ostrow's method. Bilirubin IXα dimethylester was yielded by Küster's method from the commercial bilirubin (Daiichi pure chemical Co. Ltd.), and was purified by thin layer chromatography. Photochemical natures of the fifth of six bands was coincided with that of bilirubin IXα dimethylester. The following results were obtained;<br>1. Mass spectra of bilirubin IXα dimethylester from bilirubin IX: No molecular ions were obtained by our method. The base peak at m/e 314, 300, or 227 was found and these peaks were derived from the cleavage of central methylene bridge of bilirubin IXα and IXγ dimethylester. No peaks, showing the exsistence of bilirubin IXβ and IXδ dimethylester, appeared on the mass spectrum at m/e 360 and 239.<br>2. Mass spectra of bilirubin IX dimethylester from various liver diseases and from Gunn rat: Mass spectra of the bile bilirubin from various liver diseases and Gunn rat were observed at m/e 314 or 300 as the base peak and there was no significant peaks showing other isomers such as IXβ or IXδ dimethylester. These findings coincided with that of bilirubin IXα dimethylester standard.<br>From these results, the exsistence of other isomeric compositions except bilirubin IXα (IXγ) was not able to find in the bile of human being in health and various liver diseases.

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