Human Gene for -Microseminoprotein: Its Promoter Structure and Chromosomal Localization
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- Ochiai Yukiko
- Department of Medical Biochemistry, Shiga University of Medical Science
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- Inazawa Johji
- Department of Hygiene, Kyoto Prefectural University of Medicine
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- Ueyama Hisao
- Department of Medical Biochemistry, Shiga University of Medical Science
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- Ohkubo Iwao
- Department of Medical Biochemistry, Shiga University of Medical Science
書誌事項
- タイトル別名
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- Human Gene for β-Microseminoprotein: Its Promoter Structure and Chromosomal Localization
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説明
The gene for human β-microseminoprotein (MSP, β-inhibin, prostatic secretory protein94, or PSP94) was isolated. The nucleotide sequence of its upstream region (2, 860 bp), exon 1 (35 bp), and a part of the following intron (218 bp) was determined. Transient transfection analysis on MSP-expressing (PC-3) and -nonexpressing (HepG2) cells using luciferase reporter plasmids suggested that the region from -2738 to -276 is not essential for the basal promoter activity, and that the regions from -275 to -207 and from -186 to -128 function in a cell-specific manner. The chromosome locus of the gene (MSMB) was determined through application of PCR to the DNAs of rodent-human somatic cell hybrids and also by the fluorescence in situ hybridization technique to be region q11.2 of chromosome 10.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 117 (2), 346-352, 1995
社団法人 日本生化学会
