Characterization of prostaglandin E receptor subtypes involved in the relaxation of rabbit penile corpus cavernosum smooth muscle
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- SATO Minoru
- Department of Life and Culture, Akita Keizaihouka University Junior College
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- KAWATANI Masahito
- Department of Neurohysiology, Akita University School of Medicine
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説明
Prostaglandin E (PGE) induces the penile erection in the mammalians. Receptors for PGE were characterized to 4 different subtypes (EP1—4). The effects of agonists for EP1—4 receptors were studied on phenylephrine (PE, 1 μM)-induced contractions in the rabbit penile corpus cavernosum smooth muscle. The EP4 receptor agonist, ONO-AE1-329 (1 pM—10 μM), markedly relaxed the pre-contracted smooth muscle strips in a concentration-dependent fashion (−65 ± 16% relaxation at 10 nM). The EP2 receptor agonists, ONO-AE1-259-01 (1 nM—10 μM) or butaprost (1 nM—10 μM), also relaxed the smooth muscle strips in a concentration dependent fashion (−58 ± 10% relaxation at 10 μM or −53 ± 10% relaxation at 10 μM). The EP1 receptor agonist, ONO-DI-004 (0.01—10 μM), increased the contractility in the smooth muscle strips. A 12 ± 6% increase was observed with 10 μM of the agonist. The EP3 receptor agonist, ONO-AE-248 (0.01—10 μM), however, did not alter the contractility of the smooth muscle strips. The selective EP4 receptor antagonist, ONO-AE3-208 (0.1 μM), inhibited a relaxant response to ONO-AE1-329 (the EP4 receptor agonist) but did not affect a relaxant response to ONO-AE1-259-01 (the EP2 receptor agonist). It is likely that the relaxant effect of ONO-AE1-329 acts on the EP4 receptor. Neither a nitric oxide synthase (NOS) inhibitor, L-NAME (300 μM), nor a soluble guanylyl cyclase inhibitor, ODQ (30 μM), affected the relaxant responses to ONO-AE1-259-01 (0.1—10 μM) or ONO-AE1-329 (0.01 nM—1 μM). In summary, EP4 and/or EP2 receptors mediate relaxations in rabbit corpus cavernosum smooth muscle, and the EP4 relaxant system is higher in relaxant potency than the EP2 system. Furthermore, the relaxations are independent from a nitric oxide (NO)/cGMP signalling pathway.
収録刊行物
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- Biomedical Research
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Biomedical Research 25 (5), 237-244, 2004
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詳細情報 詳細情報について
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- CRID
- 1390282679877049216
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- NII論文ID
- 130004470629
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- COI
- 1:CAS:528:DC%2BD2cXhtVGisLzF
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- ISSN
- 1880313X
- 03886107
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可