AMP N1-oxide potentiates astrogenesis by cultured neural stem/progenitor cells through STAT3 activation
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- HATTORI Noriko
- Laboratory of Molecular Biology, Gifu Pharmaceutical University Nagaragawa Research Center, Api Co. Ltd.
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- NOMOTO Hiroshi
- Laboratory of Molecular Biology, Gifu Pharmaceutical University
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- FUKUMITSU Hidefumi
- Laboratory of Molecular Biology, Gifu Pharmaceutical University
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- MISHIMA Satoshi
- Nagaragawa Research Center, Api Co. Ltd.
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- FURUKAWA Shoei
- Laboratory of Molecular Biology, Gifu Pharmaceutical University
書誌事項
- 公開日
- 2007
- DOI
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- 10.2220/biomedres.28.295
- 公開者
- バイオメディカルリサーチプレス
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説明
We earlier identified adenosine monophosphate (AMP) N1-oxide as a unique compound of royal jelly (RJ) that induces neurite outgrowth from cultured rat pheochromocytoma PC12 cells. In the present study, the effects of AMP N1-oxide on the proliferation and/or differentiation of cultured neural stem/progenitor cells (NSCs) were examined. As for cell proliferation, low micromolar concentrations of AMP N1-oxide or its parent compound, AMP, similarly enhanced the NSC proliferation-inducing activity of basic fibroblast growth factor (FGF-2), although neither compound tested alone affected cell proliferation. Conversely, high concentrations of AMP N1-oxide (over 20 μM) markedly suppressed cell growth even in the presence of FGF-2. However, this suppression was not observed with AMP. As for cell differentiation, AMP N1-oxide, but not AMP, increased the generation of astrocytes in a dose-dependent manner when the cells were cultured in medium lacking FGF-2. The generation of neurons or oligodendrocytes was not influenced by AMP N1-oxide. Furthermore, AMP N1-oxide increased the phosphorylation of STAT3 (signal transducer and activator of transcription 3), a transcription factor that mediates the expression of astrocytespecific genes. These results suggest that AMP N1-oxide is one of the components that facilitates astrogenesis by NSCs through activation of STAT3.
収録刊行物
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- Biomedical Research
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Biomedical Research 28 (6), 295-299, 2007
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