Gene expression of epigenetic regulatory factors related to primary silencing mechanism is less susceptible to lower doses of bisphenol A in embryonic hypothalamic cells

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  • Warita Katsuhiko
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University Department of Pathology, Division of Molecular Diagnostics, University of Pittsburgh School of Medicine,USA
  • Mitsuhashi Tomoko
    Department of Pathology, Division of Molecular Diagnostics, University of Pittsburgh School of Medicine,USA
  • Ohta Ken-ichi
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Suzuki Shingo
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Hoshi Nobuhiko
    Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
  • Miki Takanori
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Takeuchi Yoshiki
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

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DNA methyltransferases (DNMTs) are associated with epigenetic regulation of gene expression, and methyl-CpG binding protein 2 (MECP2) acts as a long-range regulator of methylated genes. We evaluated the effects of bisphenol A (BPA) on embryonic mouse hypothalamic cells, with particular emphasis on the gene expression of Dnmts (Dnmt1, Dnmt3a, and Dnmt3b) and Mecp2 isoforms. In a dose-dependent (0.02-200 μM BPA) 3-hr experiment, real-time reverse transcription polymerase chain reaction revealed that gene expression of both Dnmts and Mecp2_e2 was affected at 200 μM and that of Mecp2_e1 was affected at > 20 μM. These results suggest that gene expression of Dnmts and Mecp2 are less susceptible to lower doses of BPA in developing hypothalamic cells. However, as BPA concentration increases, this agent has the potential to alter gene expression of key players that provide stability and flexibility of epigenetic gene regulation, which could disrupt the normal development of hypothalamic functions.

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