Increased antibody-dependent cell-mediated cytotoxicity of spleen lymphocytes from low-dose streptozotocin-induced diabetes in mice.

Maruyama Taro, Hattori Yoshiyuki, Asaba Yoshiaki, Baba Ryoko, Ikemoto Kumiko, Takei Izumi, Taniyama Matsuo, Kataoka Kunizo, Ishii Toshiharu

doi:10.11213/tonyobyo1958.29.425

Multiple low-dose streptozotocin-induced diabetes in mice is thought to be a model of type 1 (insulin-dependent) diabetes mellitus because it is associated with insulitis. Several investigators have reported that cell-mediated immune response, especially thymic immunity, plays a critical role in the development of this type of diabetes. However, there are no reports concerning antibodydependent cell-mediated cytotoxicity (ADCC) or K-cells. Therefore, we investigated the ADCC of spleen lymphocytes from streptozotocin-induced diabetic mice and compared them with blood glucose levels and insulitis.<BR>Eight-week-old male CD-1 +/+ mice and CD-1 nu/nu mice were studied. Among the CD-1 +/+ mice, increased ADCC and insulitis were found in multiple low-dose streptozotocin-injected mice before diabetes developed and the ADCC increased with the degree of insulitis. However, there was no insulitis or increased ADCC in single high-dose streptozotocin-injected mice or control mice. Among the CD-1 nu/nu mice, increased ADCC and high blood glucose levels were found but there was no insulitis in multiple low-dose streptozotocin-injected mice.<BR>These results suggest that increased ADCC is connected with multiple low-dose streptozotocin injection and may play a pathogenetic role in the development of insulitis in the presence of thymic immunity. Our results also suggest that insulitis enhances the diabetogenic effect of streptozotocin.

Increased antibody-dependent cell-mediated cytotoxicity of spleen lymphocytes from low-dose streptozotocin-induced diabetes in mice.

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