メンブレンフィルターを応用した血漿カイロミクロン・トリグリセライドの収集定量法

DOI Web Site

書誌事項

タイトル別名
  • A colorimetric micro-determination of plasma chylomicron-triglyceride after membrane-filter filtration
  • メンブレンフィルター オ オウヨウ シタ ケッショウ カイロミクロン トリグリセライド ノ シュウシュウ テイリョウホウ

この論文をさがす

抄録

A simple method for separation of plasma chylomicron and colorimetric determination of its triglyceride content was described.<BR>Chylomicron in plasma, diluted appropriately, was collected by ultra-filtration using cellulose nitrate membrane filter with pore size of 100mμ. Chylomicron in residue was extracted with chloroform and triglyceride-glycerol was measured. The mean recovery rate of chylomicron-triglyceride was 88.6%. The mean relative error was 7.6% and there was no loss of accuracy for plasma from patients with gross hyperchylomicronemia.<BR>An analysis using paper electrophoresis revealed validity of chylomicron separation by the filtration method.<BR>The mean value of fasting chylomicron-triglyceride was 4.8±1.1 mg/100 ml (± SE) in 15 normal subjects, 12.2±2.0 mg/100 ml in 18 moderate diabetics and 30.9±5.0 mg/100 ml in 13 severe diabetics.

収録刊行物

  • 糖尿病

    糖尿病 15 (3), 175-180, 1972

    一般社団法人 日本糖尿病学会

詳細情報 詳細情報について

問題の指摘

ページトップへ