Effects of PAMAM dendrimers with various surface functional groups and multiple generations on cytotoxicity and neuronal differentiation using human neural progenitor cells

DOI Web Site Web Site PubMed 被引用文献3件 参考文献45件
  • Zeng Yang
    Center for Environmental Risk Research, National Institute for Environmental Studies Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Kurokawa Yoshika
    Center for Environmental Risk Research, National Institute for Environmental Studies
  • Win-Shwe Tin-Tin
    Graduate School of Life and Environmental Sciences, University of Tsukuba Center for Environmental Health Sciences, National Institute for Environmental Studies
  • Zeng Qin
    Center for Environmental Risk Research, National Institute for Environmental Studies
  • Hirano Seishiro
    Center for Environmental Risk Research, National Institute for Environmental Studies
  • Zhang Zhenya
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Sone Hideko
    Center for Environmental Risk Research, National Institute for Environmental Studies

この論文をさがす

抄録

Polyamidoamine (PAMAM) dendrimers have potential for biological applications as delivery systems for genes, drugs, and imaging agents into the brain, but their developmental neurotoxicity remains unknown. We investigated the effects of PAMAM dendrimers with various surface functional groups and multiple generations on neuronal differentiation using human neural progenitor cells at an equal mass concentration. Only PAMAM dendrimers containing amine (NH2) surface groups at concentrations of 10 μg/mL significantly reduced cell viability and neuronal differentiation, compared with non-amine-terminated dendrimers. PAMAM-NH2 with generation (G)3, G4, G5 G6, and G7 significantly decreased cell viability and inhibited neuronal differentiation from a concentration of 5 μg/mL, but G0, G1, and G2 dendrimers did not have any effect at this concentration. Cytotoxicity indices of PAMAM-NH2 dendrimers at 10 μg/mL correlated well with the zeta potentials of the particles. Surface group density and particle number in unit volume is more important characteristic than particle size to influence cytotoxicity for positive changed dendrimers. PAMAM-50% C12 at 1 μg/mL altered the expression level of the oxidative stress-related genes, ROR1, CYP26A1, and TGFB1, which is a DNA damage response gene. Our results indicate that PAMAM dendrimer exposure may have a surface charge-dependent adverse effect on neuronal differentiation, and that the effect may be associated with oxidative stress and DNA damage during development of neural cells.

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (45)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ