Peripheral blood monocyte separation by counterflow centrifugal elutriation.

DOI

Bibliographic Information

Other Title
  • 対向流遠心溶出法（Ｃｏｕｎｔｅｒｆｌｏｗ　Ｃｅｎｔｒｉｆｕｇａｌ　Ｅｌｕｔｒｉａｔｉｏｎ；ＣＣＥ）による血液バフィーコートからの単球分離及び分離単球の抗腫よう細胞毒性について

Search this article

Description

A method of separating human peripheral blood monocytes (HPBM) from white blood cell buffy coat by counterflow centrifugal elutriation (CCE) is described. An average of 8.7×107 HPBM, with a purify of >90%, were recovered from one unit of buffy coat processed from 450ml of whole blood. HPMB recovered by this method were viable as determined by trypan blue exclusion, had intact morphology according to light and electron microscopic observation and were not spontaneously tumoricidal as determined by a 3-day cytotoxicity assay. These HPBM could be activated to the tumoricidal state by either bacterial lipopolysaccharide (LPS) or recombinant interferon gamma (rIFN-γ)+muramyl dipeptide (MDP) and were phagocytic as judged by carbon particle or liposomal uptake. Their tumor cytotoxic function was comparable to that of HPBM separated by other methods.

Journal

Details 詳細情報について

  • CRID
    1390282679884174464
  • NII Article ID
    130003852677
  • DOI
    10.3925/jjtc1958.35.10
  • ISSN
    18838383
    05461448
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top