Determination of Activities of Lactate Dehydrogenase and α-Hydroxybutyrate Dehydrogenase in Serum Using a Membrane-Covered Plastic Formed Carbon Electrode
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- Kinoshita Hideaki
- Kwassui Women's College
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- Suda Yoshihisa
- Mitsubishi Pencil Co. Ltd.
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- Kawakubo Takamasa
- Mitsubishi Pencil Co. Ltd.
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- Usui Toshiaki
- Department of Laboratory Medicine, Nagasaki University
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- Ikeda Tokuji
- Department of Agricultural Chemistry, Kyoto University
Bibliographic Information
- Other Title
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- 新炭素素材電極を用いる血清中の乳酸脱水素酵素およびα-ヒドロキシ酪酸脱水素酵素活性の測定
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Abstract
A newly developed plastic formed carbon electrode (PFC electrode) produced a reproducible anodic current due to the oxidation of NADH at potentials less positive than those required by ordinary electrodes such as glassy-carbon electrodes. A dialysis membrane-covered PFC electrode produced steady-state currents linearly proportional to concentrations of NADH ranging from 5 to 1500μmol/l. The within-day variation for measurements was 3.1% for five runs at an NADH concentration of 100μmol/l. Activities of lactate dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (HBD) in serum were determined by measuring the decrease in current due to oxidation of NADH for 3 min at 400mV and 37°C in Tris buffer of pH 7.5 containing 350μmol/l NADH and 2 mmol/l pyrurate, or 4 mmol/l α-ketobutyrate. Serum was diluted to yield a concentration one-fifteenth that of the sample. Measurements of activities obtained using this method agreed well with those obtained using UV method (r=0.993, of LDH and HBD n=15 for LDH; r=0.999, n=15 for HBD).
Journal
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- Japanese Journal of Clinical Chemistry
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Japanese Journal of Clinical Chemistry 22 (3), 185-189, 1993
Japan Society of Clinical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679886614656
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- NII Article ID
- 130003357734
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- COI
- 1:CAS:528:DyaK2cXis1Sjtw%3D%3D
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- ISSN
- 21874077
- 03705633
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed