Studies on the Reaction of 5, 5'-dithiobis-(2-nitorbenzoic acid) with Sulfhydryl Compounds

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  • KATAOKA NAOTO
    Kochi Prefectural Caner Institute, Kochi Prefectural Central Hospital
  • AKAGI TADAATSU
    Kochi Prefectural Caner Institute, Kochi Prefectural Central Hospital
  • KOBASHI KYOICHI
    Department of Pharmaceutical Biochemistry, Faculty of Pharmaceutical Sciences, Toyama University

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  • 5, 5'-dithiobis-(2-nitrobenzoic acid)(DTNB)とSulfhydryl化合物の反応についての基礎的研究

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Ellman reported that DTNB quantitatively reacted with sulfhydryl compounds according to the following equation to form the aromatic thiol anion whose extinction coefficient was 13, 600/M/cm at 412mμ<BR>We reexamined the stoichiometry of this reaction by measuring the response curves of glutathione or cysteine with DTNB and isolated all the reaction products on paper chromatograms and Sepnadex G-10 gel filtration. From the investigation of chemical properties of the produtcs, we concluded that the mixed disulfide formed according to Eq. 1 sub- sequently reacted with a second sulfhydryl compound to give another aromatic thiol anion in the following manner.

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