HPLC法による総コレステロールおよびHDL-コレステロールの定量

DOI

書誌事項

タイトル別名
  • Quantitation of Total Cholesterol and HDL-Cholesterol by the HPLC Method: Comparison with the Automated Enzymatic Method and the Precipitation Method
  • 自動分析法および沈澱法との比較

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説明

Total cholesterol (TC) and HDL-cholesterol (C) of human sera (n=134) from subjects with serum triglyceride (TG) levels from 40mg/dl to 5378mg/dl were measured by an improved HPLC system using a gel filtration column (TSK gel Lipopropak, 7.5mm×600mm/TOSOH Co. Tokyo) and an eluent (TSKeluent LP-1). The values were compared with TC measured by the automated enzymatic method (HITACHI 7070) and HDL measured by the precipitation method. By the HPLC method, TC and HDL-C levels as well as the HDL sizes could be simultaneously and accurately obtained from the HPLC patterns monitored by cholesterol for all subjects including severe hypertriglyceridemia (n=7), in which HDL could not be separated by the precipitation method. Although the TC levels obtained by the HPLC method were consistent with those obtained by the automated enzymatic method, HDL-C levels were slightly higher than those obtained by the precipitation method. The HDL of all the samples with discrepant values between the two methods were revealed to be large in size and rich in cholesterol. Therefore, HDL subclasses with a larger size of particle and higher amount of cholesterol than normal HDL were suggested to be precipitated selectively by the precipitation method. A negative correlation was obtained between the HDL-C level by the two methods and serum TG level, but correlations were improved by excluding the samples with discrepant HDL-C levels between the two methods.

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詳細情報 詳細情報について

  • CRID
    1390282679888366336
  • NII論文ID
    130003357800
  • DOI
    10.14921/jscc1971b.25.1_28
  • ISSN
    21874077
    03705633
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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