ラット前肢粗大筋を支配する脊髄前角細胞の局在（ＨＲＰ法による研究）

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  • The Ditribution of Motoneurons Innervating the Forelimb Muscles in the Rat. A Study by use Horseradish Peroxidase(HRP).

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There are few reports about the distribution of motoneurons innervating the forelimb muscles in the rat.<br>30 Wistar rats (60 forelimbs) were used for this study.<br>We injected horseradish peroxidase (HRP) solution into their muscles which were one of deltoid or biceps, triceps, E. C. R., F. C. U., and flexor digitorums.<br>24-48 hours later, the rats were retuened by saline water, and fixed by 0.05mol PBS contained glutaraldehyde.<br>Spinal cords segments C2 to Th2 were removed, and re-fixed during 24 hours.<br>Cords were freezed, and sliced 100 micrometers thickness on the frontal plane, and reacted by diaminobentizine (DAB).<br>HRP labeled cells were counted in each slices under a microscope of 40-100 magnification.<br>Motoneurons innervating deltoid muscle located mainly in segments C5, biceps brachii in C5-6, triceps brachii in C7-8, extensor carpiradialis in C7 to Th1, flexor digitorum superficial and profunds in C8, Th1, flexor carpi ulnaris in C8, Th1.<br>Zancolli supposed and, Kendall compilated the segmental innervation of the upper extremity muscles in man.<br>The distribution innervating the forelimb muscles in the rat was similar to their reports except extensor carpi radialis.

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