Experiment on mosquito blood feeding using the artificial feeding device

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  • TSURUKAWA Chiaki
    Vector Ecology & Environment, Institute of Tropical Medicine, Nagasaki University
  • KAWADA Hitoshi
    Vector Ecology & Environment, Institute of Tropical Medicine, Nagasaki University

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Other Title
  • 人工吸血装置による蚊の吸血実験
  • ジンコウ キュウケツ ソウチ ニ ヨル カ ノ キュウケツ ジッケン

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Experiments on blood feeding of Aedes aegypti using the artificial membrane feeding system (Hemotek Ltd., Blackburn, UK), selection of good animal bloods and membranes, which were commercially available from reagent manufacturers or food companies, as good experimental materials for artificial membrane feeding of mosquitoes, were carried out. Among the 3 kinds of preserved bloods (chicken, bovine, and equine), the equine defibrinated blood, and the equine hemolyzed blood, the preserved bloods gave the best feeding results. Natural porcine gut for sausage casing was the most successful membrane as compared to the two collagen films and Parafilm®. Combinational use of the bovine preserved blood and the porcine gut membrane gave good feeding results for Ae. aegypti and Anopheles stephensi.

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