The venomous components in the worker and queen honey bees during their maturation and the seasonal generation

  • INOUE Hideo
    Laboratory of Entomology, Faculty of Agriculture, Tamagawa University:(Present address)Minophagen Research Laboratory, Minophagen Pharmaceutical Co.
  • NAKAJIMA Terumi
    Division of Molecular Biology, Institute for Medical and Dental Engineering, Tokyo Medical and Dental University:Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
  • OKADA Ichiji
    Laboratory of Entomology, Faculty of Agriculture, Tamagawa University

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Other Title
  • 日齢に伴うミツバチのハチ毒成分
  • 日齢に伴うミツバチのハチ毒成分〔英文〕
  • ニチレイ ニ トモナウ ミツバチ ノ ハチ ドク セイブン エイブン

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Compositions of the venomous principles in worker and queen honey bees (Apis mellifera) during their maturation were examined in two criteria by the pattern analysis of electrophoresis and that of high performance liquid chromatography. The venom of well matured worker bee showed electrophoretically 13 proteinous bands including those corresponding to phospholipase A_2,melittin, apamin and MCD-peptide. On the other hand, in the queen bee venom, the band corresponding to MCD-peptide was not detected but the two additional unknown components were found. The amounts of the venomous components in both worker and queen bee venoms increased remarkably within 14 days after eclosion.

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