Fundamental studies on chironomid allergy : I. Culture methods of some Japanese chironomids (Chironomidae, Diptera)

KAWAI Koichiro, KONISHI Kenichi

doi:10.7601/mez.37.47

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Other Title

ユスリカアレルギーに関する基礎的研究 : I. 日本産ユスリカ数種の培養法
ユスリカアレルギーに関する基礎的研究-1-日本産ユスリカ数種の培養法〔英文〕
ユスリカアレルギーニカンスルキソテキケンキュウ 1 ニホンサンユスリ

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Abstract

Culture methods of 3 species of chironomids widely distributed in urban areas in Japan were studied to obtain purified antigens necessary for the accurate diagnoses and desensitization therapies of hypersensitivity to chironomids. It was demonstrated that all the 3 species can be easily cultured from generation to generation in the laboratory. High yields per unit area of mature larvae of Chironomus yoshimatsui and C. flaviplumus were obtained by rearing the newly hatched larvae at densities of 8 and 4 indiv./(cm)^2,respectively, under food dose of 7.5mg/indiv. in the small containers (15cmφ×H9cm) which were spread with sand, filled with water and aerated. Fertilized egg masses of Glyptotendipes tokunagai were successfully obtained even at low adult densities in small containers when the sex ratio (♂/♀) was higher than 2/5. A considerable number of fertilized egg masses of C. flaviplumus were obtained even in a relatively small space when a time period corresponding to dusk was introduced into the illumination cycle and the illumination was reduced to 1 or 2lx in a stepwise manner at "dusk" under the sex ratio (♂/♀) of 2.

Journal

Medical Entomology and Zoology

Medical Entomology and Zoology 37 (1), 47-57, 1986

The Japan Society of Medical Entomology and Zoology

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