Detection, isolation and characterization of Orientia tsutsugamushi in Leptotrombidium intermedium
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- URAKAMI Hiroshi
- Department of Microbiology, Niigata College of Pharmacy
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- TAKAHASHI Mamoru
- Kawagoe Sogo Senior High School
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- MISUMI Hitoko
- Department of Medical Zoology, Saitama Medical School
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- OKUBO Koji
- Department of Microbiology, Niigata College of Pharmacy
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- ENATSU Teruyuki
- Department of Microbiology, Niigata College of Pharmacy
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- TAMURA Akira
- Department of Microbiology, Niigata College of Pharmacy
Bibliographic Information
- Other Title
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- アラトツツガムシ中の恙虫病リケッチアの検出, 分離, 及び分離株の性状解析
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Abstract
Unfed trombiculid mites were collected from endemic areas for detection and isolation of Orientia tsutsugamushi. The mites were compressed between a pair of slide glasses to make samples of the cuticles and smears of the tissues. Mite species were identified by observation of the cuticles, and infection with O. tsutsugamushi was examined in the tissues by improved immunofluorescent microscopy. The microorganisms were detected not only in Leptotrombidium pallidum but also in 0.17% of L. intermedium collected in Kyoto Prefecture. One strain of O. tsutsugamushi was isolated from 146 L. intermedium collected in Niigata Prefecture. Analyses of virulence to mice, antigenicity and nucleotide sequence of the 56 KDa type-specific antigen gene indicated that the isolate is closely related to a strain isolated from a patient in Niigata.
Journal
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- Medical Entomology and Zoology
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Medical Entomology and Zoology 51 (3), 169-177, 2000-09-15
The Japan Society of Medical Entomology and Zoology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679921481984
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- NII Article ID
- 110003819037
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- NII Book ID
- AN00021948
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- ISSN
- 21855609
- 04247086
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- NDL BIB ID
- 5536062
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed