Studies on the mechanisms of ionic regulation in mosquito larvae : I. The measurements of Na and Cl concentrations in haemolymph by radioactivation analysis

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  • MATSUMOTO Akira
    Department of Chemistry, Science Education Institute of Osaka Prefecture
  • MATUTANI Kozi
    Department of Biology, Science Education Institute of Osaka Prefecture

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Other Title
  • 蚊類幼虫の塩分調節機構に関する研究 : 第 1 報放射化分析による体液中のナトリウムおよび塩素濃度の測定
  • 蚊類幼虫の塩分調節機構に関する研究-1-放射化分析による体液中のナトリウムおよび塩素濃度の測定
  • カルイ ヨウチュウ ノ エンブン チョウセツ キコウ ニカンスルケンキュウ 1

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The concentrations of Na and Cl in the haemolymph of larvae of Culex pipiens pallens (Cpp), Culex pipiens molestus (Cpm), Aedes albopictus (Aalb) and Aedes togoi (At) were measured by the radioactivation analysis. The haemolymph was extracted from the thoraxes of 4th-instar larvae reared in fresh water at 23℃ for over 150 generations. One μl of haemolymph was put on a 1×1cm piece of filter paper with a capillary pipet. About 60 pieces were irradiated for 15 min in the pneumatic tube (thermal neutron flux 2.34×(10)^<13>n/(cm)^2・sec) of Kyoto University Reactor with suitable number of blank pieces and of pieces spotted by the standard NaCl solutions. Gamma rays of ^<38>Cl was measured by a pulse hight analyser soon after irradiation. About a half day later ^<24>Na was measured. No pretreatment was necessary, the measurement required less than 2min for each sample. The Na content was approximately 100mM for all species and higher than the Cl content. At had the highest Cl concentration (65mM), whereas Cpp had the lowest (40mM) (Fig.1). The changes of Na and Cl concentrations in the larval haemolymph were also studied by the treatment with artificial sea waters (Barnes' process). The Cl concentration increased faster than the Na concentration. Being in the 60% sea water for 2.5 hr Cpp had about 5.1 times of the initial Cl concentration, but Cpm had less 3.2 times. The resistance to salinity seems to be a distinctive feature between these two related species. In the 60% sea water Aalb could live after 5hr. At showed an extremely superior ability of ionic regulation. The 100% seawater caused no effect on At.

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