ヒト型精子不動化モノクローナル抗体対応抗原の局在性並びに抗体処理不動化精子の形態学的変化に関する研究

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  • Studies on localization of the antigen corresponding to human sperm immobilizing monoclonal antibody and morphological changes of the antibody treated spermatozoa.

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Human monoclonal antibody (Mab H 6-3 C 4) with strong sperm immobilizing and agglutinating activities was generated by cell fusion of human lymphocytes from a sterile woman with sperm immobilizing antibody and mouse myeloma cells and was used for immunohistochemical studies for the corresponding antigen in male genital organs. This Mab was also used for electron microscopic studies on morphological changes of spermatozoa after treatment with Mab, Immunohistochemical studies using the ABC staining system showed that the antigen epitope corresponding to MabH 6- 3 C 4 was mainly localized in epithelial layers of cauda epididymidis and seminal vesicle but not in testis, caput and corpus epididymides, or prostate. Electron microscopic observation of the positively stained cauda epididymidis showed that the antigen was densely concentrated in stereocilia of the principal cells of the epithelium. The antigen was also observed on spermatozoa in the luminal duct of cauda epididymidis and ejaculated spermatozoa. When ejaculated spermatozoa were incubated with biotinylated Mab H 6-3 C 4 and followed by the reaction with ferritin conjugated avidin for immuno electron microscopy, ferritin particles were observed on entire surfaces of sperm plasma mambrane. When ejaculated spermatozoa were incubated with Mab H 6-3 C 4 and guinea pig serum as complement, remarkable morphological changes such as peeling-out of plasma membrane or formation of numerous holes on plasma membrane were observed by scanning and transmission electron microscope.

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