Formation of HA Mediated Three-dimensional Structures by Mouse Fibroblasts due to Physical Stimulation.

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  • HIRAGAMI Fukumi
    Department of Physical Therapy, Kibi International University School of Health Science
  • KANO Yoshio
    Department of Occupational Therapy, Kibi International University School of Health Science

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  • 物理刺激による線維芽細胞の人工骨媒体三次元様増殖
  • ブツリ シゲキ ニ ヨル センイ ガ サイボウ ノ ジンコウコツ バイタイ 3ジゲン ヨウ ゾウショク

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When mixed cultures of mouse fibroblasts and hydroxyapatite (HA) were subjected to various stimuli, it was found that the fibroblasts proliferated in a three-dimensional pattern around the HA. In this study, we investigated the effects of various physical stimuli. The stimuli used were an electromagnetic field, which is thought to be effective for promotion of bone formation, electricity, ultrasonic waves and laser. Although mixed cultures of mouse fibroblasts and HA stimulated with an electromagnetic field of 1G for 16h showed only 2.8-fold higher induction rates of three-dimensional-like cell proliferation than that in the untreated control culture, stimulation for 24h and 48h resulted in 9.4-fold and 16.0-fold higher induction rates, respectively. No induction of cell proliferation was seen in the case of stimulation with electricity. Irradiation of cultures with ultrasonic waves of 5W/cm2 for 3sec resulted in no induction of cell proliferation, but irradiation for 6sec and for 9sec resulted in 4.2-fold and 4.0-fold higher induction rates, respectively. Laser irradiation of 1J/cm2 of and 5J/cm2 resulted in 6.3-fold and 3.7-fold higher induction rates, respectively, but cultures irradiated with 16J/cm2 conversely showed only 1/10 of the cell proliferation in the control culture. The results indicate that all of the types of physical stimulus tested in this study have optimal doses to induce the formation of a three-dimensional structure.

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