Interaction of the Escherichia coli Lipoprotein NlpI with Periplasmic Prc(Tsp) Protease
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- Tadokoro Akiko
- Department of Biomolecular Sciences, Faculty of Science Toho University
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- Hayashi Hidemi
- Proteome Analysis Center, Faculty of Science, Toho University Link Genomics, Inc.
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- Kishimoto Toshihiko
- Department of Biomolecular Sciences, Faculty of Science Toho University Proteome Analysis Center, Faculty of Science, Toho University
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- Makino Yasutaka
- Department of Biomolecular Sciences, Faculty of Science Toho University Proteome Analysis Center, Faculty of Science, Toho University
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- Fujisaki Shingo
- Department of Biomolecular Sciences, Faculty of Science Toho University
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- Nishimura Yukinobu
- Department of Biomolecular Sciences, Faculty of Science Toho University
書誌事項
- タイトル別名
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- Interaction of the <i>Escherichia coli</i> Lipoprotein NlpI with Periplasmic Prc (Tsp) Protease
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説明
Escherichia coli spr (s-uppressor of prc) mutants and nlpI mutants show thermosensitive growth. The thermosensitivity of the spr mutants was suppressed by the nlpI mutations. Expression of the fusion genes encoding hexa-histidine-tagged NlpI (NlpI-His) and purification of the tagged NlpI showed that NIpI-His bound with Prc protease and IbpB chaperone. NlpI-His with the amino acid substitution of G103D did not bind with either of these proteins, while NlpI-His variants (NlpI-284-His, NlpI-Q283-His, and NlpI-G282-His) lacking 10 to 12 residues from the carboxy terminus bound with both proteins. The tagged NlpI lacking 11 amino acid residues from the carboxy terminus was processed by Prc, but that lacking 12 residues was not. The thermosen-sitivity of the nlpI mutant was corrected by the production of the former NlpI variant, but not by production of the latter. Expression of the truncated NlpI that lacked 10 or 11 residues from the carboxy terminus corrected the thermosensitivity of the prc nlpI double mutant, while expression of the full-length NlpI did not. Thus, it was suggested that NlpI was activated by Prc protease processing.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 135 (2), 185-191, 2004
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679940636160
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- NII論文ID
- 130003534652
- 10016198110
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2cXktV2hu7o%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 6887594
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- PubMed
- 15047720
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可