EFFECT OF CULTURE SUPERNATANTS OF PHA-STIMULATED MONONUCLEAR CELLS UPON BASOPHIL HISTAMINE RELEASE IN ATOPIC PATIENTS

Muto Kazuhiko, Kasei Masaharu, Ito Shigeru, Oshida Yoshihiro, Mukai Mikio, Hiratani Michio

doi:10.15036/arerugi.29.257

We investigated the effect of culture supernatants from PHA-stimulated mononuclear cells on antigen (Dermatophagoides farinae)-induced histamine release from leukocytes in atopic patients. Histamine release was estimated by enzyme-isotopic assay. The antigen-induced histamine release was not affected by the addition of PHA and in the absence of antigen, the PHA-stimulated culture supernatants of mononuclear cells could not release histamine from leukocytes of atopic patients. As a whole, the culture supernatants of mononuclear cells stimulated with PHA of various concentrations, whether obtained from atopic patients or from non-atopic subjects, exerted similar enhancing activity on the antigenstimulated histamine release of atopic patients. However, when stimulated with 3μg/ml concentration of PHA-P, the culture supernatants from atopic patients showed a higher enhancing activity on histamine release than did those obtained from non-atopic controls (p<0.02). The maximum enhancing effect of culture supernatants seemed to be correlated with their RAST count to D.farinae (r=0.42, NS). These results suggest that under some conditions, soluble factors released from mononuclear cells of atopic patients may exert a pronounced enhancing effect on basophil histamine release by sensitized antigens as compared with those released from control mononuclear cells.

EFFECT OF CULTURE SUPERNATANTS OF PHA-STIMULATED MONONUCLEAR CELLS UPON BASOPHIL HISTAMINE RELEASE IN ATOPIC PATIENTS

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