Fc-Receptors on Human B-Lymphocytes : Fc-Rosette Inhibition by Anti HLA and Anti β_2-Microlobulin

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  • Morito Tadao
    Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo
  • Tanimoto Kiyoaki
    Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo
  • Hashimoto Yoshimi
    Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo
  • Horiuchi Yoshihiko
    Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo
  • Juji Takeo
    Department of Transfusion, School of Medicine, University of Tokyo

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Other Title
  • ヒトリンパ球のFcレセプターに関する研究 : 抗HLA血清および抗β_2ミクログロブリンによるFcロゼット阻止
  • ヒト リンパキュウ ノ Fc レセプター ニカンスルケンキュウ コウ HLA

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Abstract

One of B-cell specific alloantigens controlled by the gene locus in I-region of H-2 complexes was recently disclosed to be detectable by serological methods in mice. The antigens are called Ia-antigen (I-region associated antigen), and thought to be Ir-gene products. It was recently suggested that the antigen is identical or closely related to Fc-receptor on B-lymphocytes in mice. In human, even the presence of Ia-antigen is uncertain but it is possible that anti HLA sera may contain anti Ia like activities. In the present study, twelve anti HLA sera were used for the inhibition of Fc rosette formation and some of them imhibited the rosette formation without any relation of HLA type specificities of antisera and HLA haplotypes of used lymphocytes. The antisera retained most of inhibitory activities after the absorption with type-corresponding T-lymphocytes. Reduction and alkylation or sedimentation by ultracentrifugation of the anti HLA sera resulted in no marked reduction of the activities. These results indicated that the activity is independent from either immune complexes nature or anti HLA activity, and that it is B-cell specific and closely related to Fc receptor suggesting anti-Ia like activity in mice. It is well established that β_2 microglobulins compose a part of the HLA antigen molecule. Rabbit antiserum to human β_2-microglobulin markedly and uniformly inhibited the Fc-rosette formation, despite the different HLA type sources of lymphocytes. This suggested us that Fc-receptors have close relation with β_2-microglobulin and that a part of their molecules might be conposed of β_2-microglobulins like HLA antigens.

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