BIOCHEMICAL ANALYSIS OF TRANSFER FACTOR

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  • Transfer Factorの生物化学的性質
  • Transfer Factor ノ セイブツ カガクテキ セイシツ

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Abstract

We reported earlier that the ultrafiltrated extracts (M.W.<5000) of peripheral leukocytes from tuberculin-sensitive donors contain the materials (transfer factor, TF) which enable nonsensitive recipients to gain the ability to react to this antigen. The crude ultrafiltrate was then subjected to gel filtration on a Sephadex G-10 column to fractionate the active components. The present study was undertaken to determine whether the fractionated materials may specifically transfer the donor's sensitivity to a nonsensitive recipient and whether chemotactic activity exists in these materials. The elute from Sephadex G-10 was assayed for polypeptide content by the fluorescamine reagent (Fluram) and 4 Fluram reactive peaks (i.e. G-1, G-2 (subfractions F-G-2 and B-G-2), G-3 and G-4) were noted. The administration of B-G-2 or G-3 fraction to a tuberculin nonreactive recipient could elicit a reaction to this antigen. Moreover, by using the modified Boyden-chamber method and in vivo skin tests, these fractions were shown to have no chemotactic activity to neutrophils.

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