STUDIES ON THE MECHANISMS OF TUBERCULIN HYPERSENSITIVITY II. Non-dialyzable Transfer Factor

DOI Web Site PubMed

Bibliographic Information

Other Title
  • ツベルクリンアレルギーに関する研究　ＩＩ　　受身伝達因子（ＴＦ）
  • ツベルクリン アレルギー ニカンスルケンキュウ 2 ウケミ デンタツ インシ

Search this article

Description

There have been numerous reports on the transfer factor (TF) of delayed-type hypersensitivity, but there are still unsolved problems about its mechanisms. The experiments reported herein wereperformed in order to clarify the transfer mechanisms of tuberculin-type hypersensitivity (TTH). Usedas a TF was the non-dialyzable fraction of the extract obtained from spleen cells of guinea pigs vaccinated and challenged (VC group) with heat-killed BCG.<BR>1. A fraction which showed TTH-transfer activity was isolated from the spleen extract of the VC group by column chromatography using a Sephadex G-75. It's molecular weight was 15, 000- 67, 000.<BR>2. The low molecular fraction of TF did not contain H37Rv antigen or an antibody to H37Rv.<BR>3. Recipient animals intravenously injected with the TF showed a positive skin reaction to PPDon the 3rd day, but the reaction was negative one week after the injection.<BR>4. TTH was transferred to normal recipients with thymocytes or spleen cells from normal animalsincubated with TF in vitro for 24 hours, but not with the lymphnode cells of those animals.<BR>It was demonstrated that TTH could be transferred to normal recipients by thymocytes orspleen cells treated with TF in vitro. This results suggests that normal T cells are sensitized throughthe same process in recipient animals injected with TF.

Journal

Details 詳細情報について

Report a problem

Back to top