結核菌検査室内Ｃｒｏｓｓ‐ｃｏｎｔａｍｉｎａｔｉｏｎの１事例

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タイトル別名
  • A CASE OF LABORATORY CROSS-CONTAMINATION OF MYCOBACTERIUM TUBERCULOSIS ON THE BROTH-BASED CULTURE SYSTEM
  • ケッカクキン ケンサシツナイ Cross contamination ノ 1 ジレイ

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抄録

We experienced a case of laboratory cross-contamination of Mycobacterium tuberculosis on the broth based culture system. These false-positive cultures were confirmed by analysis of DNA fingerprinting, RFLP method, which showed the same pattern in three specimens with that of the first manipulated specimen in our laboratory on that day, out of 7 specimens examed. We found possible several process causing cross-contamination where mixture of the foreign body could occur in buffer or NALC-NaOH. False-positive cultures for Mycobacterium tuberculosis may lead to unnecessaly, potentially toxic, costly treatment, and changing the treatment strategy. So we must critically interpret a single positive culture, especially by liquid media.

収録刊行物

  • 結核

    結核 82 (11), 825-829, 2007

    一般社団法人 日本結核病学会

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