ＡｌＦ分子吸光法によるプラーク中総フッ素の定量

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  • Total fluoride determination in dental plaque by AlF molecular absorption spectrometry.

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Total fluoride (F) determination in dental plaque is rather complicated because of its small quantities and low F concentration smapled from the individual. Full dissociation of the bound F in dental plaque is also required for the assay by F sensitive electrode.<BR>Recently, AlF (aluminum monofluoride) molecular absorption spectrometry was developed for the ultratrace analysis of F. An application of this method to the determination of total F in dental plaque was investigated.<BR>The results were as follows:<BR>1) The AIF absorbance was linearly increased by increase of F (5-50ng/ml) added to sample solutions (0.5-2.1mgdry plaque/ml). The regression coefficients of sample solutions were almost equal to that of the standard solution. The sensitivity was 0.066ng F per 20μl standard solution at the 1% absorption level, and the relative detection limit was 0.0036ppm (3.6ppb).<BR>2) The variations of absorbance increased with the plaque amounts, but the recovery of fluorine from sample solution was not affected by the amounts of plaque contained.<BR>3) The coefficient of variation of F concentration in small plaque samples (approximately 1mg dry weight) ranged from 8.6 to 15.9%, and F concentrations of five pooled plaque samples ranged from 8.0 to 12.3ppm (dry weight).<BR>These experiments suggest that AlF molecular absorption spectrometry is useful for analysis of total F in dental plaque, especially in small samples and at low F concentrations.

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