フッ素濃度100ppmおよび250ppmのフッ化物洗口液のエナメル質におよぼす影響について

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  • Effects of fluoride mouth-rinsing solution at 100ppm and 250ppm of fluoride on human enamel.

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The purpose of this study was to assess the fluoride uptake by human enaml from dilute fluoride mouth-rinsing solution and the acid solubility of the enamel, by means of the window method and electron probe microanlysis.<BR>Experiments were done in vitro by treatment with phosphoric acid-acidified NaF solutions (100ppm of F-; pH5.0, 250 ppm of F-; pH5.0) as the mouth-rinsing solutions. The fluoride content in successive layers of enamel was determined with an ORION ion meter and fluoride electrode. The calcium content was determined by atomic absorption spectrophotometry. An acid solubility test was used to determine the calcium released from enamel into a 0.4M acetate-sodium acetate buffer (pH4.0) every thirty minutes during a 3-hr period. Electron probe microanalysis was performed to determine the distribution of F, Ca, and P in fluoride-treated human enamel surface.<BR>The fluoride content of human enamel increased with time. Especially the surface layer of enamel contained a greater amount of fluoride. The distribution of fluoride in enamel treated with the 250 ppm fluoride mouth-rinsing solution was greater than that of the 100ppm fluoride mouth-rinsing solution. The acid solubility test proved that the acid resistance was greater in fluoride treated enamel than in the control. Electron probe microanalysis showed that fluoride was taken up at the depth of approximate 60-90μm.<BR>As a result, we suggest that the repeated use of dilute fluoride mouth-rinsing solutions over a long period will effectively prevent caries, .

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