SIMULTANEOUS ESTIMATION OF PHENOL AND INDOLE IN THE PUTREFIED HUMAN SALIVA

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  • TSUDA Michiko
    Department of Biochemistry, Nippon Dental College Department of Biochemistry, School of Medicine, Tokushima University Faculty of Education, Ibaraki University

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Other Title
  • 腐敗唾液のフェノール・インドール同時定量法 (紫外部光吸収法)
  • フハイ ダエキ ノ フェノール インドール ドウジ テイリョウホウ シガイブ ヒカリ キュウシュウホウ

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The ether extract of incubated saliva (several days incubation in an aerobic condition) contains only one or two compounds, phenol or phenol and indole, as light absorbents in the ultraviolet region. This paper deals with the determination of these compounds.<BR>The optical density of the extract was proportional to the concentration of phenol or indole in the material (Figs. 9 and 10). Each 3 ml of the materials, saliva and standard solutions, was extracted with each 6 ml of ether or petroleum ether. The optical densities (O.D.) of these extracts were estimated at 2872 Å in the case of petroleum ether (P.E.) and at 2730 Å in the case of ether (E.). Procedures were summarized in next column. When the concentration of indole or phenol in the standard solution is 1mg/100 ml water, the content of phenol or indole in saliva can be calculated by the following equations;<BR>Indole (I): a/c mg/100 ml saliva<BR>Phenol (P): b-d·I/e mg/100 ml saliva<BR>In this method the recovery was 99-103% (Table 7) and the relative error 1.9% (Table 8).

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