抗酸菌の臨床細菌学的同定に関する一考察

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書誌事項

タイトル別名
  • A STUDY ON THE IDENTIFICATION OF CLINICALLY SIGNIFICANT MYCOBACTERIA
  • コウサンキン ノ リンショウ サイキンガクテキ ドウテイ ニ カンスル 1 コ

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説明

In view of an increasing demand for simple methods of identification of various mycobacteria for clinical use, our seven years' experiences were summarized and the results were evaluated to find out a better method.<BR>A total of 263 strains of mycobacteria, which were either isolated in our Institute or referred to our laboratory from several other institutes, have been submitted to differential identification. The key differential features which we have adopted were (1) acid-fastness, (2) cell forms on smear, (3) speed of growth, (4) characteristics of colony (rough or smooth), (5) pigmentation at dark, (6) photoreactive pigmentation, (7) growth on Sabouraud's agar and nutrient agar, (8) PAS degradation, (9) Tween 80 hydrolysis (5 days), (10) nitrate reduction, (11) semiquantitative catalase, (12) arylsulfatase, (13) ami dases (urease and pyrazinamidase) and (14) niacin. The mycobacterial strains could be differentiated into six species and four groups, namely, Mycobacterium tuberculosis, M. kansasii, M. marinum, M. scro fulaceum, other scotochromogens, M. intracellulare-avium complex, other nonphotochromogens, M. fortuitum, M. chelonei and other rapid growers. Further differentiation into species among each group was assessed to be not accurate enough due to our relatively simple identification system, however the system seemed to be valid as far as our present clinical situation concerns.<BR>The validity of our identification system was again evaluated in this study using forty seven docu mented strains, nearly all of which were kindly provided by the U.S.-Japan Cooperative Medical Science Program-NIAID in 1975 (Trudeau Mycobacterial Culture Collection=TMC strains). Eleven key differential features were adopted in this reevaluation, and the seventh, twelfth and thir teenth keys were excluded because of either relative complexity of the technique or paucity of differ ential features in view of our present clinical situations.<BR>Our second simplified system revealed to be still valid for the defferentiating various mycobacteria into six species and four groups.<BR>Shortcomings of our differentiation system seem to be in case of identification of M. xenopi and M. szulgai which will be probably differentiated into “other scotochromogens” and also in case of niacin negative M. tuberculosis as well as M. africanum, though the former two species are almost negligible in their frequency in our clinical isolates.

収録刊行物

  • 結核

    結核 54 (10), 481-490, 1979

    一般社団法人 日本結核病学会

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