COMPARATIVE EVALUATION OF ACID-FAST STAINING FOR THE DETECTION OF <i>MYCOBACTERIUM FORTUITUM</i>
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- YOSHIDA Shiomi
- Clinical Research Center
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- TSUYUGUCHI Kazunari
- Clinical Research Center
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- SUZUKI Katsuhiro
- Department of Respiratory Medicine
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- TOMITA Motohisa
- Department of Clinical Laboratory, National Hospital Organization Kinki-chuo Chest Medical Center
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- OKADA Masaji
- Clinical Research Center
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- HAYASHI Seiji
- Department of Respiratory Medicine
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- ARIKAWA Kentaro
- Kobe Institute of Health
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- IWAMOTO Tomotada
- Kobe Institute of Health
Bibliographic Information
- Other Title
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- <i>Mycobacterium fortuitum</i>を対象としたZiehl-Neelsen染色法と蛍光染色法における抗酸性の比較検討
- Mycobacterium fortuitumを対象としたZiehl-Neelsen染色法と蛍光染色法における抗酸性の比較検討
- Mycobacterium fortuitum オ タイショウ ト シタ Ziehl-Neelsen センショクホウ ト ケイコウ センショクホウ ニ オケル コウサンセイ ノ ヒカク ケントウ
- ─ Clinical Performance of Fluorescent and Ziehl-Neelsen Staining ─
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Abstract
<p>[Objective] Fluorescent staining is of paramount importance, not only for confirming the presence of mycobacteria in a given specimen but also for providing an estimated growth quantification. In this study, for rapidly growing Mycobacterium fortuitum, we evaluated the effectiveness of a rapid fluorescent staining method employing auraminerhodamine (AR) fluorescent stain and acridine-orange (AO) fluorescent stain compared to that of the standard Ziehl-Neelsen (ZN) stain currently in use in our laboratory. [Method] We evaluated the acid-fast nature of M.fortuitum strain ATCC6841 and 42 clinical isolates from each patient diagnosed at NHO Kinki-chuo Chest Medical Center. These isolates were preliminarily identified as M.fortuitum using DNA-DNA hybridization (DDH Mycobacteria; Kyokuto Pharmaceutical, Tokyo, Japan). These isolates were further identified by comparative sequence analysis of the ITS regions and the partial 16S rRNA gene. [Results] A total of 26 M.fortuitum strains (61.9%) demonstrated the lack of an acid-fast nature by AR staining, and slightly fewer demonstrated the same by AO staining. Sequence analysis of these 42 clinical isolates led to the identification of 35 M.fortuitum subsp. acetamidolyticum isolates (83.3%) and 7 closely M.fortuitum isolates. [Discussion] This work reported the loss of the acid-fast nature of specific M.fortuitum strains. It is likely that both the specific cell envelope of M.fortuitum and the staining mechanics could have been responsible for the loss of the acid-fast nature since the 2 different fluorescent stains yielded the same results. M.fortuitum is a mycobacterium species that does not stain with the commonly used fluorescence microscopy technique. Therefore, we suggested the use of an identification scheme for these organisms that employs ZN staining and the study of cultural characteristics (growth rate, temperature, and pigment production).</p>
Journal
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- Kekkaku(Tuberculosis)
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Kekkaku(Tuberculosis) 88 (5), 461-467, 2013
JAPANESE SOCIETY FOR TUBERCULOSIS
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Keywords
Details 詳細情報について
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- CRID
- 1390282679980276096
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- NII Article ID
- 130005415090
- 10031176472
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- NII Book ID
- AN00073442
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- ISSN
- 18842410
- 00229776
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- NDL BIB ID
- 024668038
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed