Effects of Toothpastes Containing Either Dipotassium Glycyrrhizinate, Dipotassium Glycyrrhizinate with Create Ion^[○!R] High Power, or Dipotassium Glycyrrhizinate with Create Ion^[○!R] Number One, and the Effects of These Pastes Combined with Mechanical Stimulation of Gingiva on Periodontitis in the Rat Model

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  • YAMAMOTO Tatsuo
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • EKUNI Daisuke
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • YAMANAKA Reiko
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • SAKAMOTO Tomonori
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • HAYASHI Hironori
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
  • WATANABE Tatsuo
    Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

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Other Title
  • ラット歯周炎モデルに対するグリチルリチン酸ジカリウム, クレイツイオン^[○!R]を配合した歯磨剤と機械的刺激との効果
  • ラット歯周炎モデルに対するグリチルリチン酸ジカリウム,クレイツイオンを配合した歯磨剤と機械的刺激との効果
  • ラットシシュウエン モデル ニ タイスル グリチルリチンサン ジ カリウム クレイツイオン オ ハイゴウ シタ ハミガキザイ ト キカイテキ シゲキ ト ノ コウカ

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Abstract

The objective of this study was to compare the effects of four pastes-regular toothpaste as a control (CT), regular toothpaste containing either dipotassium glycyrrhizinate (GLY), dipotassium glycyrrhizinate with Create Ion^[○!R] High Power (HP), or dipotassium glycyrrhizinate with Create Ion^[○!R] Number One (No 1), on periodontitis in the rat model. In addition, the effects of mechanical stimulation of gingiva when combined with the pastes were also evaluated. Under general anesthesia, lipopolysaccharide and bacterial protease solution were applied once a day into the palatal gingival sulcus of the upper right and left first molars of 40 male Wistar rats to induce periodontitis for 8 weeks. From the 5th to 8th week, 4 toothpastes (n=10) were applied in the gingivae of the right and left maxillary first molar once per day for 10 minutes respectively. Also, the palatal gingiva of the left molar was stimulated once a day using a power toothbrush. After 8 weeks, the teeth and gingivae were fixed, decal-cified and embedded in paraffin. Buccolingual sections were evaluated using hematoxylin-eosin staining. Statistical differences among the 4 groups and between stimulated and non-stimulated sides were tested by the ANOVA or Kruskal-Wallis test, and the Student's t-test or Mann-Whitney test, respectively. In the No 1 group, the number of polymorphonuclear leukocytes in the junctional epithelium was significantly decreased. In the HP group, the number of polymorphonuclear leukocytes in connective tissues was significantly less than that in the other groups. The stimulated side had a lower number of polymorphonuclear leukocytes in the junctional epithelium and connective tissue than the non-stimulated side in the GLY group, and the values were similar to those for the stimulated sides of the HP and No 1 groups. In conclusion, Create Ion^[○!R] High Power and Create Ion^[○!R] Number One with dipotassium glycyrrhizinate reduces polymorphonuclear leukocyte infiltration into connective tissues and junctional epithelium, respectively. Mechanical stimulation of gingiva combined with dipotassium glycyrrhizinate reduces polymorphonuclear leukocytes in junctional epithelium and connective tissue as strongly as dipotassium glycyrrhizinate and Create Ion^[○!R] without mechanical stimulation.

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