Measurement of the Total Number of Bacteria in Saliva Using Quantitative Real-time PCR (qPCR) : Evaluation of the oral hygiene status
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- YOKOYAMA Masaaki
- Oral Health Management Center, Tokushima University Hospital
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- FUKUI Makoto
- Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
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- MASUDA Kaname
- Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
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- TAKAMATSU Natsuko
- Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
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- OKADA Jurou
- Kagawa Dental Association
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- TAKEBE Hiromitsu
- Kagawa Dental Association
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- KATAOKA Kosuke
- Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
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- ITO Hiro-O
- Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
Bibliographic Information
- Other Title
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- リアルタイム定量PCR(qPCR)法による唾液中の総細菌数の測定 : 口腔清潔度の指標としての試み
- リアルタイム テイリョウ PCR qPCR ホウ ニ ヨル ダエキ チュウ ノ ソウ サイキンスウ ノ ソクテイ コウクウ セイケツド ノ シヒョウ ト シテ ノ ココロミ
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Description
The state of oral hygiene has been evaluated by employing various indices, such as the Oral Hygiene Index, Plaque Index, Patient Hygiene Performance, and Plaque Control Record (O'Leary's PCR). Measuring the scores of these indices is time- and labor-consuming, requiring manual reading and recording by professionals, standardization of examination methods. In contrast, measuring the total number of bacteria in saliva using quantitative real-time PCR (qPCR) allows the straightforward collection and storage of samples, making it possible to measure many samples at the same time. This technique facilitates the quantification of the number of bacteria within a shorter time than the culture method. In the present study, the total number of bacteria measured by qPCR was found to be significantly decreased (p<0.05) in stimulated whole saliva collected from 5 volunteers by chewing paraffin after tooth cleaning. These findings were in agreement with the decreases shown by Greene's Debris Index and O'Leary's PCR values. There was a significant correlation (r=0.55, p<0.01) between the salivary bacterial counts measured by qPCR from 81 subjects before and after a 4-month interval. These findings suggest that it is possible to measure the total number of bacteria in saliva using qPCR. This technique is convenient and is suggested to be applicable for assessing the oral hygiene status in the future.
Journal
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- JOURNAL OF DENTAL HEALTH
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JOURNAL OF DENTAL HEALTH 59 (3), 183-189, 2009
Japanese Society for Oral Health
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Keywords
Details 詳細情報について
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- CRID
- 1390282679981008896
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- NII Article ID
- 110008460531
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- NII Book ID
- AN00081407
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- ISSN
- 21897379
- 00232831
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- NDL BIB ID
- 10398297
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed