Measurement of the Total Number of Bacteria in Saliva Using Quantitative Real-time PCR (qPCR) : Evaluation of the oral hygiene status

DOI Web Site 22 References
  • YOKOYAMA Masaaki
    Oral Health Management Center, Tokushima University Hospital
  • FUKUI Makoto
    Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
  • MASUDA Kaname
    Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
  • TAKAMATSU Natsuko
    Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
  • OKADA Jurou
    Kagawa Dental Association
  • TAKEBE Hiromitsu
    Kagawa Dental Association
  • KATAOKA Kosuke
    Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
  • ITO Hiro-O
    Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School

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Other Title
  • リアルタイム定量PCR(qPCR)法による唾液中の総細菌数の測定 : 口腔清潔度の指標としての試み
  • リアルタイム テイリョウ PCR qPCR ホウ ニ ヨル ダエキ チュウ ノ ソウ サイキンスウ ノ ソクテイ コウクウ セイケツド ノ シヒョウ ト シテ ノ ココロミ

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Abstract

The state of oral hygiene has been evaluated by employing various indices, such as the Oral Hygiene Index, Plaque Index, Patient Hygiene Performance, and Plaque Control Record (O'Leary's PCR). Measuring the scores of these indices is time- and labor-consuming, requiring manual reading and recording by professionals, standardization of examination methods. In contrast, measuring the total number of bacteria in saliva using quantitative real-time PCR (qPCR) allows the straightforward collection and storage of samples, making it possible to measure many samples at the same time. This technique facilitates the quantification of the number of bacteria within a shorter time than the culture method. In the present study, the total number of bacteria measured by qPCR was found to be significantly decreased (p<0.05) in stimulated whole saliva collected from 5 volunteers by chewing paraffin after tooth cleaning. These findings were in agreement with the decreases shown by Greene's Debris Index and O'Leary's PCR values. There was a significant correlation (r=0.55, p<0.01) between the salivary bacterial counts measured by qPCR from 81 subjects before and after a 4-month interval. These findings suggest that it is possible to measure the total number of bacteria in saliva using qPCR. This technique is convenient and is suggested to be applicable for assessing the oral hygiene status in the future.

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