Successful Collection of Peripheral Blood Stem Cells Mobilized by a Combination of G-CSF with High-Dose Ara-C or High-Dose Etoposide.

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  • KUZUYAMA Yuko
    The Department of Hygiene, Kyoto Prefectural University of Medicine The Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • SONODA Yoshiaki
    The Department of Hygiene, Kyoto Prefectural University of Medicine
  • SAKABE Hideaki
    The Department of Hygiene, Kyoto Prefectural University of Medicine
  • NAKAGAWA Hitoshi
    The Third Department of Internal Medicine Kyoto First Red Cross Hospital
  • FUJII Hiroshi
    The Third Department of Internal Medicine Kyoto First Red Cross Hospital
  • ABE Tatsuo
    The Department of Hygiene, Kyoto Prefectural University of Medicine

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Other Title
  • Ｇ‐ＣＳＦを併用したＡｒａ‐Ｃ大量療法，Ｅｔｏｐｏｓｉｄｅ大量療法による末梢血幹細胞の効率的採取法
  • G-CSFを併用したAra-C大量療法,Etoposide大量療法による末梢血幹細胞の効率的採取法
  • G-CSF オ ヘイヨウシタ Ara-C タイリョウ リョウホウ Etopos

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Description

The author evaluated the effectiveness of a combination of G-CSF with high-dose Ara-C (HD Ara-C) or high-dose etoposide based on the collection of peripheral blood stem cells (PBSC) during the recovery phase from myelosupression in 17 patients with various types of hematopoietic malignancies. Following therapy with HD Ara-C or HD etoposide, G-CSF (250μg/body) was administered from the nadir state and blood mononuclear cells were collected using a CS-3000 by processing 15 litres of blood at each apheresis. Stem cell-enriched fractions obtained from discontinuous Percoll gradients were collected and stored in liquid nitrogen. The mean numbers of granulocyte-macrophage colony-forming units (CFU-GM) obtained by the first apheresis were 10.9×105/kg after chemotherapy with HD Ara-C and 13.4×105/kg after HD etoposide therapy, respectively. In 14 out of the 17 first apheresis, more than 2×105 CFU-GM/kg were collected. Serial apheresis was performed in seven patients, at intervals ranging from 21∼79 days, and the progenitor yield in the second apheresis decreased in five patients. These results suggest that the use of either HD Ara-C or HD etoposide induces an effective mobilization of PBSC and enables large scale collection of PBSC for autotransplantation.

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 34 (12), 1525-1531, 1993

    The Japanese Society of Hematology

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