低浸透圧液に対する急速溶血反応の定速注水法による自動記録式分析の一新方法

DOI Web Site PubMed

書誌事項

タイトル別名
  • A New Simple Method for the Analysis of Hemolytic Reaction by Automatic Recording with Continuous Dilution Device
  • 低浸透圧液に対する急速溶血反応の定速注水法による自動記録式分析の1新方法--微量定速注水式赤血球抵抗測定法について-1-
  • テイ シントウアツエキ ニ タイスル キュウソク ヨウケツ ハンノウ ノ テイ
  • —微量定速注水式赤血球抵抗測定法について　第1報—
  • —A Micro-method for the Measurement of Erythrocyte Osmotic Resistance by Continuous Dilution Procedure 1st Report—

この論文をさがす

説明

A new simple method for the analysis of hemolytic reaction by automatic recording with a pouring water device was developed and applied successfully to the measurement of human erythrocyte osmotic resistance. Five μl of whole blood was added to 500 μl of barbital buffered saline in a small test tube placed in a platelet aggregation meter (EEL). Warmed distilled water was poured into the tube through a capillary plastic tubing at a constant rate and the changes in optical density at 660 nm was recorded automatically.<br>Saline concentration after T seconds in the tube (Ct) was calculated by the following equation: Ct=CoVo÷(Vo+RT), Co: initial conc. of saline, Vo: initial volume of saline, R: rate of pouring water. A typical pattern of the dilution hemolysogram (DHG) showed a reversed S shape, and the points of initial, 50% and complete hemolysis were estimated from this DHG.<br>This new micro-method was proved to be a simple, rapid and reproducible procedure for the accurate measurement of erythrocyte osmotic resistance.

収録刊行物

  • 臨床血液

    臨床血液 22 (2), 178-185, 1981

    一般社団法人 日本血液学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ