Methylcholanthreneによつてシロネズミに生じた紡錘形細胞肉腫の組織培養(予報)
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- Notes on a tissue culture clone of a methylcholanthrene-induced spindle-cell sarcoma of the rat
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The general character of the tissue culture clone of a methylcholanthrene-induced spindle-cell sarcoma was described in the present paper. The spindle-cell sarcomas were induced in 16 rats out of 20 which received, twice at a 15-day interval, 0.5cc of 0.5 per cent methylcholanthrene dissolved in olive oil. A tissue culture clone was established from one of rats bearing the spindle-cell sarcomas. The roller tube method was adopted for tissue culture; pieces of the tumor tissue were planted on cover slips in clots consisting of equal parts of fowl plasma and 50 per cent chicken extract, and fed with 2ml of culture medium composed of 2 parts of balanced salt solution (Tyrode's or Gey's), 2 parts of horse serum and 1 part of 50 per cent chicken extract. The medium was renewed 3 times a week. The subculture was made every 7 or 8 days.<br>After the 8th subculture (53 days after being transferred in vitro), the culture material was returned to a new rat subcutaneously. The rat which received the tumor injection produced the spindle-cell sarcoma with the nature similar to the original tumor. In the tissue culture material, two kinds of cells, one is a spindle-shaped and the other is a round-shaped cell, were observed. The chromosome investigation in the tissue culture material indicated that there exist at least two populations characterized by near-diploid and near-tetraploid cells respectively.
収録刊行物
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- 山階鳥類研究所研究報告
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山階鳥類研究所研究報告 1 (10), 410-412, 1957
公益財団法人 山階鳥類研究所
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詳細情報 詳細情報について
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- CRID
- 1390282680015158400
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- NII論文ID
- 130003572515
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- ISSN
- 18833659
- 00440183
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- データソース種別
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- JaLC
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