Transplantation of Cryopreserved Mouse, Chinese Hamster, Rabbit, Japanese Monkey and Rat Ovaries into Rat Recipients
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- KAGABU Satosi
- Department of Veterinary Science, Faculty of Agriculture, Yamaguchi University
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- UMEZU Motoaki
- Laboratory of Animal Reproduction, Tohoku Graduate School of Agricultural Science, Tohoku University
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抄録
Partial ovaries from mice, hamsters, rabbits, Japanese monkeys and rats have survived deep-freezing and returned to a normal morphological state after being thawed and transplanted into the rat uterine cavity. This report describes the ice-free cryopreservation of mouse and other ovaries at -196°C by vitrification. The vitrification solution was based on the solutions reported by Rall & Fahy [16]. After ovaries had been exposed to the vitrification solution, they were frozen, with their suspending medium, by liquid nitrogen. After freezing, the ovaries were thawed in 37°C water. The viability of the previously frozen ovarian tissue was tested by transplanting it into the uterine cavity of pseudopregnant rats. Seven days after transplantation, the ovaries were removed with the rat uterus, and stained with haematoxylin and eosin for histological examination. Survival of the frozen-thawed the ovaries in the rat uterine cavity demonstrates that these ovaries can tolerate exposure to osmotic dehydration and vitrification in a concentrated solution of cryoprotectant and are then immunologically acceptable to the uterine cavity.<br>
収録刊行物
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- Experimental Animals
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Experimental Animals 49 (1), 17-21, 2000
公益社団法人 日本実験動物学会
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詳細情報 詳細情報について
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- CRID
- 1390282680017677440
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- NII論文ID
- 120000860075
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- NII書誌ID
- AA11032321
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- COI
- 1:STN:280:DC%2BD3c3mt1OitQ%3D%3D
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- ISSN
- 18817122
- 00075124
- 13411357
- http://id.crossref.org/issn/13411357
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- NDL書誌ID
- 4960248
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- PubMed
- 10803357
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可