Contractility of Single Myofibrils of Rabbit Skeletal Muscle Studied at Various MgATP Concentrations.
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- Wakayama Jun'ichi
- Department of Physics (Biophysics Section), Faculty of Science, Science University of Tokyo
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Abstract
A novel experimental method was developed to study the contractility of single myofibrils of skeletal muscle. Single myofibrils (ca. 1 μm in diameter) prepared from glycerinated rabbit psoas muscle were suspended between rigid and flexible microneedles by the entwining method. The length changes of the preparations applied via the rigid microneedle by an actuator and the force produced were measured by photo-electrically detecting the nanometer deflections of the flexible microneedle. Single myofibril preparations maintained uniform sarcomere striations during contraction-relaxation cycles. The isometric force produced, the velocity of unloaded shortening, and the force-velocity relationship of single myofibrils were investigated at various MgATP concentrations. The contractility of single myofibrils thus obtained in the absence of ATP regenerative systems was essentially the same as that of skinned muscle fibers under comparable conditions in the presence of ATP regenerative systems. Thus, it was found that (1) the present experimental method is useful for studying the contractility of single myofibrils, and (2) in single myofibril preparations, the MgATP concentration at actomyosin sites is well equilibrated with that in bathing solutions.<br>
Journal
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 50 (5), 533-542, 2000
THE PHYSIOLOGICAL SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390282680018471808
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- NII Article ID
- 130004435742
- 10008292188
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- NII Book ID
- AA00691224
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- COI
- 1:CAS:528:DC%2BD3cXptVejsrw%3D
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- ISSN
- 18811396
- 0021521X
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- NDL BIB ID
- 5591323
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- PubMed
- 11120920
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed