Relationship between Intracellular Calcium and Airway Reactivity in Guinea Pigs.
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- Jain Deepika
- Department of Biochemistry, Vallabhbhai Patel Chest Institute, Delhi University
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The present study was carried out to examine the relationship between intracellular free calcium ion concentrations and its regulatory enzymes, sodium potassium adenosine triphosphatase (Na+,K+-ATPase) and calcium adenosine triphosphatase (Ca2+-ATPase), with airway reactivity to inhaled histamine in guinea pigs. Forty-nine guinea pigs were included in this study. Of these, 34 animals responded to histamine bronchoprovocation challenge in vivo with a greater than 35% fall in specific airways conductance and were labeled as "reactive," and the remaining 15 were "nonreactive." The dose of histamine producing a 35% fall in specific airways conductance was labeled as ED35 SGaw. The animals were then sacrificed, and the following biochemical measurements were carried out: intracellular free calcium ion concentrations [Ca2+]i in leukocytes and isolated tracheal smooth muscle cells, activities of Na+,K+-ATPase and Ca2+-ATPase in tracheal homogenate, and plasma levels of lysophosphatidylcholine (LPC). Reactive guinea pigs showed significantly higher [Ca2+]i and Na+,K+-ATPase and Ca2+-ATPase activities. Airway reactivity (ED35 SGaw) had significant negative correlation with [Ca2+]i, with activities of each of the ATPases and with plasma lysophosphatidylcholine. It is concluded that the level of [Ca2+]i is an important determinant of airway reactivity. Intracellular calcium levels modulate airway response to histamine with higher levels being associated with greater reactivity.<br>
収録刊行物
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 51 (5), 577-583, 2001
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680018525056
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- NII論文ID
- 130004435791
- 10008294818
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- NII書誌ID
- AA00691224
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- COI
- 1:CAS:528:DC%2BD38XjvVCitA%3D%3D
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- ISSN
- 18811396
- 0021521X
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- NDL書誌ID
- 6003295
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- PubMed
- 11734078
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可